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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has actually been noticed in many tumors, which results in chromosome instability, and therefore may possibly add to tumorigenesis. This protein was discovered to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a significant position in advertising mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have already been explained. Two more splice variants have been described but specific nucleotide sequence data is not still readily available.
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Blocking peptides are peptides that bind exclusively to the target antibody and block antibody binding. These peptide typically consists of the epitope recognized via the antibody.
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This guidebook outlines additional feasible optimization of cellular and immunoassay parameters to make certain the absolute best outcomes are...
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This guide outlines more doable optimization of mobile and immunoassay parameters to make sure the best possible outcomes are...
Antibodies certain to the blocking peptide not bind on the epitope to the concentrate on protein. By evaluating the staining with the blocked antibody vs . the antibody by yourself, you can see which staining is particular.
Focus on Information and facts Cyclin E belongs for the really conserved cyclin household, whose users are characterized by website a remarkable periodicity in protein abundance throughout the mobile cycle. Cyclins functionality as regulators of CDK kinases. Diverse cyclins exhibit unique expression and degradation designs which add for the temporal coordination of each mitotic occasion. Cyclin E kinds a fancy with and features to be a regulatory subunit of CDK2, whose action is needed for mobile cycle G1/S changeover. This protein accumulates in the G1-S phase boundary and is also degraded as cells development by S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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